SciEn [さ]

updated on 2019/06/23

[さ]

とびら

あ

か

さ

た

な

は

ま

や

ら

わ

数

雑

さ	最近の	・In a recent study, we demonstrated that [B]
	最大／最小	・The contamination by oral epithelium was considered to be minimal because the layer of ameloblast-lineage cells is separated by alveolar bone from the oral epithelium in the rat incisor. ・When maximum staining had develop, cells were rinsed with water to stop the reaction.
	最適の条件	・To determine the optimum effective concentration of HGF
	さらに	・The explants that had been cultured for 3 days with AS-ODN and then culturedfurther for 11 days withour AS-ODN showed no cusp formation. <further cultured ではない> ・Three-day AS-ODN treatment of the tooth germ explants obtained from E13.5 mice inhibited the formation of the enamel knot, and furtherculture of the explants in the absence of AS-ODN resulted in the lack of cusps, .....
	算出する	・The number of cells was correlated with the absorbance by reference to a blank control, and the cell number was calculated from following formula: cell numebr (cells.well) = 4.1051x10⁵xA₅₅₀-8799.5. =細胞数はブランク試料をリファレンスにして得られた吸光度と関係があり、その数は次式により求められた。<数式が入っても最後にピリオドを打って文の体裁をとること; calculate は式から「計算する」 [B]> ・This index was calculated from the mean percent of scattering cells in ten 0.25-mm² fields per well. ・a scattering index was estimated by counting individual cells. <estimate は数えたりして「合計を出す」[B]>
し	しかし	・However, the mechanism remains to be clarified. = しかしながら、そのメカニズムは未だ不明である（解明されるべく残されている）。[B]
	支配する	→制御する
	示す	・6 ng/ml appeared to be the optimum effective concentration in cultures. = 示適濃度であることを示した。[B] ・Immunohistochemical analysis demonstrated the characteristic temporal and spatial distribution of HGF. ・These results all indicate that ... ・Our study in culture also showed that ... ・as indicated in the Results = Results で述べた様に・This implies that .... ・These results suggest that BMP4 is necessary for the formation of the enamel knot and that the latter subsequently induces the cusp formation in the tooth germ. ・Two alternative transcriptional starting sites were identified, one in exon I and the other in exon II and the alternative splicing is regulated mainly by the 5' flanking region of exon I. ・Resulting exon combinations were defined as I-III-IV-V and II-III-IV-V. =となる
	謝辞	・The authors thank Dr.T.Nakamura (XXX Univ, Japan) for providing rabbit antiserum against rat HGF. <We thanks よりも The authors の方が謝辞にふさわしい(らしい) [B]> ・Part of this study was supported by a Grant for Scientific Research from the Japanese Ministry of Education, Science, and Culture (project 064xxxx). <a part of ではない。大文字の場所に注意>
	周辺の	・the surrounding mesenchyme = その周辺にある間葉 [B] ・the mesenchymal area near the cervical loop = CL近くの間葉 [B] ・the dental pappillae adjacent to the cervical loop = CLに隣接する [B]
	条件下で	・Total RNAs were isolated from explants cultured for 14 days under various conditions. <in various... ではない [M]> ・Transcripts for G3PDH were detected under all conditions. <in any conditions ではない> ・...., phosphorothioated ODN itself used in our study seemed to have no toxic effect under the present conditions. <presence の時は in the presence of となる。in と underをきちんと使い分けること [M]>
	調べる	→明らかにする
	知られている	・HGF, known also as SF = SF としても知られている <also known as ではない [B]>
す	すなわち	→つまり
	～する	・Note that, although IEE cells in the occlusal region in non-treated control and RS-ODN-treated explants had differentiated into preameloblasts (B, E), those cell in AS-ODN-treated explant did not undergo the differentiation (H). ・mesenchymal growth induced by BMP4 occurs via the dental epithelium.
せ	制御する	・Two alternative transcriptional starting sites were identified, one in exon I and the other in exon II and the alternative splicing is regulated mainly by the 5' flanking region of exon I.
	精製する	・Antibodies were purified by use of highly purified recombinant rat HGF that had been immobilized on Sepharose beads.
	設計する	・It was analyzed by use of OLIGO to design the antisense construct.
	全体で	・in the entire area of dental papillae [B] ・Positive signals are still detectable throughout the enamel organ
そ	阻害する	・blockage of the translation of the mRNA for HGF by an antisense oligodeoxynucleotide..[B] ・To investigate the role of BMP4 in tooth development, we used AS-ODN to cause BMP4 translation arrest in vitro. ・Three-day AS-ODN treatment of the tooth germ explants obtained from E13.5 mice inhibited the formation of the enamel knot, and further culture of the explants in the absence of AS-ODN resulted in the lack of cusps, ..... ・Tucker et al. (1998) suggested that Noggin, which binds BMP4 with high affinity and can abolish its activity by blocking the binding of it to cell surface receptors, transformed tooth identity from incisor to molar shape. <「うち消す」abolish と「阻害する」 blocking の使い分け。>
	促進する	→促す
	染まる	・the cells that had incorporated BrdU were stained blue-black upon immunostaining with HRP-conjugated BrdU-specific antibodies and subsequent treatment with the appropriate substrates. ・When maximum staining had develop, cells were rinsed with water to stop the reaction. ・Positive signals are still detectable throughout the enamel organ
	存在／非存在	→在る

とびらへ

前へ　次へ

↑　トップへ